Funded Proposals

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TnT Funded Proposals

The TnT Program funds upgrade and equipment purchases for existing cores, the purchase of shared equipment made available within departments, and the start-up costs for new core facilities.

Below is a list of recently funded proposals for:
New Cores and Services
New Shared Instruments
Equipment Upgrades for Existing Cores
 

New Cores and Services:

HSPH Bioinformatics Core (HBC)

In an effort to provide bioinformatics analysis services and training to the HMS Community, the HMS Tools and Technology Program has provided additional support and resources to the HSPH Bioinformatics Core (HBC). HBC provides expertise in areas such as array analysis, next-gen sequencing (NGS) and functional analysis. Their NGS support includes epigenetics, transcriptomics and re-sequencing studies. HBC works together with research computing groups on all aspects of data management.
Contact: Meeta Mistry
Visit the core's website.

Longwood Medical Area CyTOF Core

The Longwood Medial Area (LMA) CyTOF Core Facility uses innovative technology available to the Harvard Medical School Community that integrates Mass Spectrometry with Flow Cytometry. The heart of the facility is a CyTOF®2 Mass Cytometer that is capable of analyzing, at the single cell level, up to 40 markers simultaneously with minimal signal overlap. The facility has both a dedicated operator and a data analyst who assist investigators as they design assays, process samples, and analyze the high dimensional data produced by the CyTOF. For more information and to access the core facility please contact the CyTOF Operator.
Contact: Nicole Carlson, MS, CyTOF Specialist
Email: nicole_carlson@dfci.harvard.edu
Phone: 617-632-6561
Visit the core's website.

The Image and Data Analysis Core (IDAC)

IDAC provides image data analysis services to individual labs and core facilities at Harvard Medical School, including the ICCB-Longwood Screening Facility (ICCB-L), the Drosophila RNAi Screening Center (DRSC), and the Nikon Imaging Center (NIC).
Facility Directors: Hunter Elliott, PhD and Tiao Xie, PhD
Visit the core's website.

HMS Mouse Imaging Core

The HMS Mouse Imaging Core provides access for researchers to in-vivo mouse imaging for endpoint and longitudinal studies using either luminescent or fluorescent reporters in an Animal Biosafety Level 2 environment.   Staff from the ICCB-Longwood Screening Facility oversee the IVIS (In-Vivo Imaging System) Lumina II mouse imager and its use, while the IVIS room is operated and maintained by the HCCM (Harvard Center for Comparative Medicine).  Staff members provide access and training for the imager and data analysis computers, while the HCCM oversees all room and animal operations including facility access, safety training and orientation, animal transfers and husbandry, and protocol approval.
Facility Contact: iccbl_ivis@hms.harvard.edu; or 617-432-5815 (the ICCB-Longwood Screening Facility)
Visit the core's website.

The Thermo Fisher Center for Multiplexed Proteomics at Harvard Medical School

The Thermo Fisher Center offers proteome-wide protein expression profiling. The Center uses recently-introduced high resolution mass spectrometers (Orbitrap Fusion Tribrid) and isobaric tandem mass tags (TMT) to quantify protein expression from cells or tissues. Currently the tandem mass tag technology allows for the analysis of 6-10 samples within a single experiment.  For example, protein expression in a cancer cell line can be profiled after nine drug treatments and compared to an untreated sample across >6,000 proteins.  The services are provided to the HMS community as well as external users.
Contact: Ryan Kunz, PhD, Associate Director
Visit the core's website.

The Research Instrumentation Core Facility

The Research Instrumentation Core Facility enables the development of new scientific instruments in order to further research at Harvard Medical School and affiliated institutions.  The facility provides consultations on the design of hardware or software, purchasing decisions, and assistance with implementation.  The staff will work with researchers to develop and fabricate customized electronic, optical, and mechanical devices, as well as software interfaces for these devices.  Furthermore, the facility provides tools and workshop space for authorized researchers to design and prototype their own instruments.  The facility is staffed by a full-time Neurobiology device engineer and a staff engineer. 
Contact: Ofer Mazor, PhD, Director
Email: ofer_mazor@hms.harvard.edu
Visit the core's website.

Centralized Mechanism for Scientific Software Support

SBGrid currently supports the structural biology community by collecting, maintaining, and installing the scientific software necessary for research. TnT funding will allow us to expand this service to other disciplines throughout Harvard Medical School. In the first stage of this activity, a comprehensive collection of scientific software supported by SBGrid will be made accessible to all members of the HMS community on the RITG Orchestra Cluster. Access to software collection will be extended to scientific workstations at HMS laboratories as the project develops.
Faculty Director: Piotr Sliz, PhD
Visit the Core's website.

Genome Engineering Production Group

The Genome Engineering Production Group (GEPG) was established in 2013 with the goal of facilitating genome engineering-based research through the availability of reasonably priced tools. The GEPG currently offers custom TALEN and CRISPR nuclease constructs for use in engineering human and mouse cells, as well as for model organisms including zebrafish, C. elegans, and Drosophila.  We also offer TALE-based transcriptional activator constructs.
Contact: Gerald Marsischky, PhD
Visit the core's website.

 

New Shared Instruments:

KinTek RFQ-3 Quench-Flow Device

For analysis of enzymatic reactions on short time scales.
Departments: Cell Bio/BCMP
Contact: Dan Finley, PhD
Email: daniel_finley(at)hms.harvard.edu

Acumen eX3/HCI (High Content Imager)

Bench-top laser scanning imaging cytometer; cell-based high content screening for multicolor, multiplexed assays.
Core: ICCB-Longwood Screening Facility
Contact: Jennifer Smith, PhD, Assistant Director
Visit the core's website.

Pall ForteBio Octet RED384

Label-free system to quantify and monitor biomolecular reactions (binding affinities, on-off rate kinetcs, protein/small molecule/ nucleic acids.)
Core: ICCB-Longwood Screening Facility
Contact: Jennifer Smith, PhD, Assistant Director
Visit the core's website.

PerkinElmer EnVision Multiplate Reader

Core: ICCB-Longwood Screening Facility
Contact: Jennifer Smith, PhD, Assistant Director
Visit the core's website.

Fluodigm C1 Single-Cell AutoPrep Instrument

Automated single-cell isolation and preparation system for genomic analysis.
Core: Biopolymers Facility
Contact: Robert Steen, Director
Visit the core's website.

Baker BioProtectIV Class II A2 Biosafety Cabinet

Adds new capacity to sort human and infected cells/clinically relevant samples.
Core: Systems Biology FACS Facility
Contact: Jodi Moore, PhD, Director
Visit the core's website.

 

Equipment Upgrades for Existing Cores:

Drosophila RNAi Screening Center/ICCB-Longwood Screening Facility

Upgrade for the Evotec Opera confocal high-throughput microscope.
Contact: Stephanie Mohr, PhD, Director
Visit the core's website.

The Nikon Imaging Center at Harvard Medical School

Upgrade for the spinning disk confocal microscope.
Contact: Jennifer Waters, PhD, Director
Visit the core's website.

Biopolymers Facility

Upgrade for two Illimuna HiSeq 2500, SPRI robot for library prep, Qiagility high precision robot for qPCR prep, Sorvall Centrifuge.
Contact: Robert Steen, Director
Visit the core's website.

The Nikon Imaging Center at Harvard Medical School

Green technology for Imaging Facility.
Contact: Jennifer Waters, PhD, Director
Visit the core's website.

Neurobiology Imaging Facility

Upgrade for Olympus Slide-scanning Microscope- only scope of its kind on campus.
Contact: Michelle Ocana, Facility Manager
Visit the core's website.

Drosophila RNAi Screening Center/ICCB-Longwood Screening Facility

Funds for Perkin Elmer Opera Microscope service contract.
Contact: Stephanie Mohr, PhD, Director
Visit the core's website.

ICCB-Longwood Screening Facility

New Whole Genome siRNA Library for ICCB-Longwood.
Contact: Jennifer Smith, PhD, Assistant Director
Visit the core's website.

Faculty & Staff

Spotlight

Jennifer Smith, PhD, Assistant Director, ICCB-Longwood

 

 

I joined ICCB-Longwood in 2011 after completing a post-doctoral fellowship here at Harvard Medical School.  Throughout my educational training, I utilized viruses to better understand basic principles in molecular and cellular biology.  Now I have the opportunity to share my screening experiences and continue to learn with investigators that use ICCB-L to perform small molecule and RNAi screens. When not at ICCB-L, I can be found enjoying the outdoors here in New England.

 

Learn more about the ICCB-Longwood Screening Facility