Events and Announcements
FEATURED EVENT: The Power of Multiplexed Proteomics
The Power of Mulitplexed Proteomics
When: Wednesday, May 28, 2014 | 2:00-4:00pm
Where: Armenise Amphitheatre
*Reception to follow in Modell Atrium
Steve Gygi, PhD
Marc Kirschner, PhD
Mike Weekes, MD, PhD
Bruce Spiegleman, PhD
ANNOUNCEMENT: Schedule an information session with the NEW HSPH Bioinformatics Core.
There is a new bioinformatics core available to researchers at HMS. In an effort to provide bioinformatics analysis services and training to the HMS Community, the HMS Tools and Technology Program has provided additional support and resources to the HSPH Bioinformatics Core (HBC).
Want to learn more?
HBC Information Sessions: HBC can deliver presentations at departmental meetings or lab meetings. The presentation would be 10-15 minutes and would provide a brief overview of the core and what kind of support they provide.
If you are interested in scheduling an information session or would like to learn more please contact Meeta Mistry, Bioinformatics Analyst at: email@example.com
PAST EVENT: New Genome Engineering Core Overview at Mammalian RNAi Screeners Group Meeting
Mammalian RNAi Screeners Group Meeting
When: Thursday, March 20, 2014 | 10:00-11:00am
Where: Warren Alpert Building, Room 563
"The Genome Engineering Production Group (GEPG): a new source of low-cost CRISPR and TALE tools for the HMS research community"
Stephanie Mohr, PhD - Director of DRSC, HMS
GEPG website: http://gepg.hms.harvard.edu/
"A 'how-to' tour of the Online GESS told for prediction of miRNA-like off-target effects in RNAI screen data by seed region analysis"
Bahar Yilmazel, MS - Drosophila RNAi Screening Center (DRSC), HMS
DRSC website: http://www.flyrnai.org/
This meeting is composed of two short presentations about new resources available to the HMS community. The first will highlight the new Genome Engineering Production Group (http://gepg.hms.harvard.edu/), which designs and builds custom CRISPT sgRNA and TALE nuclease expression constructs. The second talk will be the adaptation of the GESS algorithm initially designed by Frederic Sigoillot and Randy King (Nature Methods, 2012, 9(4):363-366, PMID: 22343343) into an online GESS tool accessible from the DRSC website.
NEW CORE: The Thermo Fisher Scientific Center for Multiplexed Proteomics at Harvard Medical School
The new Thermo Fisher Scientific Center at Harvard Medical School offers proteome-wide protein expression profiling. The Center uses recently-introduced high resolution mass spectrometers (Orbitrap Fusion Tribrid) and isobaric tandem mass tags (TMT) to quantify protein expression from cells or tissues. Currently the tandem mass tag technology allows for the analysis of 6-10 samples within a single experiment. For example, protein expression in a cancer cell line can be profiled after nine drug treatments and compared to an untreated sample across >6,000 proteins. The services are provided to the HMS community as well as external users.
Please visit the website at http://tcmp.hms.harvard.edu for more information and details on submitting samples.
NEW CORE: The Longwood Medical Area CyTOF Core Facility is open for use.
The Longwood Medial Area (LMA) CyTOF Core Facility uses innovative technology available to the Harvard Medical School Community that integrates Mass Spectrometry with Flow Cytometry.
The heart of the facility is a CyTOF®2 Mass Cytometer (pictured above) that is capable of analyzing, at the single cell level, up to 40 markers simultaneously with minimal signal overlap. The facility has both a dedicated operator and a data analyst who assist investigators as they design assays, process samples, and analyze the high dimensional data produced by the CyTOF.
NEW CORE: Visit ICCB to use the IVIS (In-Vivo Imaging System) Lumina II mouse imager.
The HMS Mouse Imaging Core provides access for researchers to in-vivo mouse imaging for endpoint and longitudinal studies using either luminescent or fluorescent reporters in an Animal Biosafety Level 2 environment. Staff from the ICCB-Longwood Screening Facility oversee the IVIS (In-Vivo Imaging System) Lumina II mouse imager and its use, while the IVIS room is operated and maintained by the HCCM (Harvard Center for Comparative Medicine). Staff members provide access and training for the imager and data analysis computers, while the HCCM oversees all room and animal operations including facility access, safety training and orientation, animal transfers and husbandry, and protocol approval.
High-throuput chemical and RNAi screening
This symposium featured a number of brief talks outlining high-throughput screening technologies and resources available throughout the Harvard community.
Find out more HERE.
HMS Core Facilities Talk and Poster Session
The TnT Committee hosted a poster session and a series of talks to highlight the services, facilities and shared equipment available to researchers on the Quad.
Find out more HERE.